African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Toxicity assessment of modified Cry1Ac1 proteins and genetically modified insect-resistant Agb0101 rice

Hyun Suk Cho
  • Hyun Suk Cho
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Myung Ho Lim
  • Myung Ho Lim
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Si Myung Lee*
  • Si Myung Lee*
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Yun Soo Yeo
  • Yun Soo Yeo
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Sung Duk Oh
  • Sung Duk Oh
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Seon Woo Oh
  • Seon Woo Oh
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Soo Yun Park
  • Soo Yun Park
  • Division of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Soon Jong Kweon
  • Soon Jong Kweon
  • Division of Central Area Breeding, National Institute of Crop Science, Rural Development Administration, Jeonju, Republic of Korea.
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Seok Cheol Suh
  • Seok Cheol Suh
  • Division of Molecular Breeding, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea.
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Soon Ki Park
  • Soon Ki Park
  • School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea.
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  •  Received: 10 April 2015
  •  Accepted: 18 May 2015
  •  Published: 10 June 2015

Abstract

Insect-resistant Agb0101 rice was recently developed by modifying the cry1ac1 gene by changing codon usage changes relative to the native truncated cry1ac1 gene. To assess the toxicity of genetically modified Agb0101 rice, we conducted bioinfomational comparisons of the amino acid sequences that are not similar to known toxic proteins. Sufficient quantities of mCry1Ac1 protein were produced in Escherichia coli for in vitro evaluation and animal study. We compared the amino acid sequences and molecular mass. There have the same amino acid sequences and molecular masses after purifying the modified Cry1Ac1 (mCry1Ac1) protein from highly expressed bacteria and genetically modified rice were identical. We also investigated the acute and 90-days oral toxicities. No adverse effects were observed in mice following acute oral exposure to 2,000 mg/ kg body weight mCry1Ac1 protein of body weight and 90 days oral exposure to Agb0101. These results indicate that mCry1Ac1 proteins and Agb0101 rice demonstrate no adverse effects in these tests when applied via gavage and feed, respectively.

 

Key words: Modified Cry1Ac1, food safety assessment, toxicity, insect- resistant rice Agb0101.

Abbreviation

ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BUN, urea nitrogen; TG, triglyceride; A/G Ratio, albumin/globulin ratio; IP, inorganic phosphorus.