African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12365

Full Length Research Paper

Genetic structure of populations of Mugil cephalus using RAPD markers

E. Suresh1*, V.K.Tiwari1, M. Sekar2, M. Sankar1 and A. Kathirvelpandian3
1Central Institute of Fisheries Education, Mumbai - 400 061, India. 2Presently in Central Marine Fisheries Research Institute, Vizhag, India 3Presently in National Bureau of Fish Genetic Resources, Kochi Unit, India.
Email: [email protected]

  •  Accepted: 28 October 2013
  •  Published: 30 October 2013

Abstract

Genetic structure of four populations of Mugil cephalus from Gujarat, Maharashtra, Andhra Pradesh and Tamil Nadu in India was studied using randomly amplified polymorphic DNA (RAPD) markers. Five selective primers provided distinct and consistent RAPD profiles in all the four populations. The bands in the range 400 to 1200 bp were scored for consistent results. The RAPD profiles generated by all the five primers revealed varying degrees of polymorphism, ranging from 50.76 (primer E03) to 72.41% (primer E05). Nei’s genetic diversity (h) among the four populations varied from 0.3717 ± 0.1460 (Gujarat population) to 0.5316 ± 0.1720 (Maharashtra population). Nie’s highest genetic distance (0.8556) was observed between Tamil Nadu and Gujarat populations.

 

Key words: Mugil cephalus, randomly amplified polymorphic DNA (RAPD), genetic structure, India.

Abbreviation

Abbreviations: RAPD, Random amplified polymorphic DNA; PCR, polymerase chain reaction; EDTA, ethylenediaminetetra acetate; SDS, sodium dodecyl sulfate.