African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Effect of different growth parameters on chitinase enzyme activity of acridine orange and ethidium bromide mutant bacteria of the gut environment

H. Ann Suji
  • H. Ann Suji
  • Faculty of Marine Sciences, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu 608002, India.
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T. A. Palavesam
  • T. A. Palavesam
  • Center of Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tirunelveli, Tamil Nadu, India.
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G. Immanuel
  • G. Immanuel
  • Center of Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tirunelveli, Tamil Nadu, India.
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Suthin Raj*
  • Suthin Raj*
  • Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram – 608 002, India.
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  •  Received: 12 December 2013
  •  Accepted: 06 May 2014
  •  Published: 04 June 2014

Abstract

The degradation of chitin is mediated primarily by bacterial chitinases. Bacteria produce several chitinases, probably to hydrolyze the diversity of chitins found in nature. Chitinases have shown numerous applications in waste treatment and management of shellfish processing industries. Therefore, in the present study, an attempt was made to optimize chitinase production by one of the shrimp’s gut mutant bacterial flora, Pseudomonas alcaligenes. Optimization of culture conditions revealed that the enzyme production was maximum in pH 7.5 (107.4 ± 0.50 U/ml), temperature 35°C (103.15 ± 1.74 U/ml) when the carbon and the nitrogen sources used were CMC (106.0 ± 1.89 U/ml) and KNO3 (91.2 ± 1.51 U/ml), respectively. The total chitinase production for all optimum conditions is 105.07 ± 1.33 U/ml.

 

Key words: Chitinase, shrimp gut, mutant, Pseudomonas alcaligenes, optimization condition.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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