African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Determination of petroleum-degrading bacteria isolated from crude oil-contaminated soil in Turkey

  Esin Eraydin ErdoÄŸan1*, Fikrettin Åžahin2 and Ayten Karaca1        
  1Department of Soil Science, Faculty of Agriculture, Ankara University, 06110 Diskapi – Ankara, Turkey. 2Department of Genetics and Bioengineering, Faculty of Architecture and Engineering, Yeditepe University, Kadiköy - Istanbul, Turkey.
Email: [email protected]

  •  Accepted: 15 April 2011
  •  Published: 13 March 2012

Abstract

 

In order to establish an experimental basis for bioremediation of soil contaminated with crude oil, 33 strains of bacteria with hydrocarbon-degrading ability were isolated from the contaminated soil in Adana, Batman and Adiyaman, Turkey. The strainswere identified as Pseudomons spp., Paucimonas lemoignei, Stenotrophomonas maltophiliaEschericha spp., Enterobacter spp. Citrobacter koseri, Acinetobacterspp., Aeromonas caviae, Sphingobacterium multivorum, Klebsiella pneumoniae.Pseudomons aeruginosa, Pseudomonas putida biotype A, Citrobacter amalonaticusGC subgroup A and Acinetobacter genomospecies, respectively. The ability to utilize crude oil as carbon source for their growth was ascertained. These bacterial isolates obtained in this study have catabolic capabilities for the biodegradation of petroleum hydrocarbons. Further study under soil conditions may be necessary to determine biodegradation potential of bacterial mixture tested in contaminated soil.

 

Key words: Soil, crude oil, soil pollution, bacteria, biodegradation