African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12489

Genetic diversity analysis of various red spider mite-resistant upland cotton cultivars based on RAPD

Zhaohui Liu1, 2, Yong Zhang1, 3*, Shuo Zhang1, Kejun Deng1, Shunwen Dong2and  Zhenglong Ren1, 3*  
1School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu, 610054, P. R. China. 2Industrial Crop Breeding and Cultivation Institute, Sichuan Academy of Agriculture Sciences, Jianyang, 641400, P. R. China. 3Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Ya’an, 625014, P. R. China.  
Email: [email protected]; [email protected]

  •  Accepted: 04 March 2011
  •  Published: 02 May 2011

Abstract

This study is carried out to analyze the genetic diversity of red spider mite-resistant cotton (Gossypium hirsutum) cultivars that are applied in cultivar identification and breeder's right protection of cottons. The genomic DNA was used as template and random primers were used to analyze the genetic diversity of 21 accessions ofGossypium hirsutum by RAPD-PCR. Among the 100 primers screened, 20 primers could generate 176 fragments, 96.02% of which were polymorphic ones. The similarity coefficient of cultivars was between 0.2273 - 0.9773. The average values of effective number of alleles, Nei’s gene diversity and Shannon’s information index were 1.7391, 0.4017, and 0.5773, respectively. Cluster analysis based on UPGMA revealed that 21 cultivars could be divided into three groups. The analysis revealed that it was corresponding to the geographical distribution and most of the lines had a wide genetic base.

 

Key words: Cotton, red spider mite, RAPD, genetic diversity, cluster analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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