African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Screening of recombinant inbred lines for salinity tolerance in bread wheat (Triticum aestivum L.)

Amin Azadi1*, Eslam Majidi Hervan2, Seyed Abolghasem Mohammadi3, Foad Moradi2, Babak Nakhoda2, Mojtaba Vahabzade4 and Mohsen Mardi5  
1Department of Plant breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran. 3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tabriz University, Tabriz, Iran. 4Seed and Plant Improvement Institute, Karaj, Iran. 5Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Email: [email protected]

  •  Accepted: 08 August 2011
  •  Published: 05 October 2011

Abstract

Screening and increasing of salinity tolerance of crops is an important aim of many plant breeders. Screening a large number of plants for salinity tolerance is not easy, therefore this investigation was performed to evaluate and screen 186 F8recombinant inbred lines (RILs) derived from a cross between Superhead#2 (Super Seri) and Roshan wheat varieties for salinity tolerance. All the individuals were evaluated under two treatments including control (10 mM NaCl) and salt stress (150 mM NaCl). Different traits relating to salt tolerance, including dry matter of shoot and root, sodium and potassium concentration, chlorophyll content and relative water content (RWC) were measured. Significant differences were observed among wheat RILs for all the measured traits. Seedling growth was reduced by salinity in all RILs and we observed a significant negative correlation between shoot dry matter and sodium concentration (r = -0.52**), whereas significant positive correlations between shoot dry matter and K+/Na+ ratio (r = 0.36**), chlorophyll content (r = 0.20**) and RWC (r = 0.39**) were detected. Based on our results, sodium concentration of leaf, K+/Na+ ratio, chlorophyll content and RWC are good indexes for screening bread wheat genotypes for salinity tolerance.

 

Key words: Screening, salinity tolerance, recombinant inbred lines, wheat.

Abbreviation

RILs, Recombinant inbred lines; RWC, relative water content; ST,salinity tolerance.