African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Outcrossing rate in olive assessed by microsatellite and inter simple sequence repeat (ISSR) markers

Rafaeli Aparecida Vieira de Souza1, Juliano Lino Ferreira2, Francyane Tavares Braga2, Patrícia Helena de Azevedo1, Gustavo César Sant’Ana2, Ana PaulaRibeiro2, Aluízio Borém3 and Geraldo Magela de Almeida Cançado2*  
1Tropical Agriculture Department, Federal University of Mato Grosso (UFMT), 78060-900, Cuiabá, Mato Grosso, Brazil. 2Plant Biotechnology Laboratory, Agricultural Research Agency of Minas Gerais (EPAMIG), 37780-000, Caldas, Minas Gerais, Brazil. 3Agriculture Department, Federal University of Viçosa (UFV), 36570-000, Viçosa, Minas Gerais, Brazil.  
Email: [email protected]

  •  Accepted: 28 May 2012
  •  Published: 03 July 2012

Abstract

Olive is known to be an allogamous species. The aim of this study was to estimate the magnitude of cross-pollination rate using microsatellite simple sequence repeats (SSR) and inter simple sequence repeats (ISSR) molecular markers in olive genotypes. The DNA from maternal plants and 23 progenies of two different accessions, Ascolano USA and MGS GRAP541 were extracted and screened with two microsatellite and ten ISSR markers. The outcrossing rate and other related parameters were analyzed using the MLTR application. The set of estimates, individually and collectively, support the hypothesis of frequent allogamy in both olive genotypes evaluated, with high rates of outcrossing in both markers.

 

Key words: Olea europaea L., outcrossing rate, zygotic embryos, mating system, molecular markers.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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