African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Effect of different concentrations of carbon source, salinity and gelling agent on in vitro growth of fig (Ficus carica L.)

Issam M. Qrunfleh1*, Mohamad M. Shatnawi2 and Zakaria I. Al-Ajlouni3
  1Department of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa’ Applied University, Al-Salt 19117, Jordan. 2Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa’ Applied University, Al-Salt 19117, Jordan. 3Department of Plant Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid 22110, Jordan.
Email: [email protected]

  •  Accepted: 15 February 2013
  •  Published: 27 February 2013

Abstract

 

Three in vitro experiments were conducted to study the effect of different concentrations of carbon source, salinity and gelling agent on number of new shoots, shoot length, fresh weight, and dry weight of fig (Ficus carica L.) to develop an efficient in vitro propagation method. The results show that there were no significant differences between the three carbon sources regarding number of newly formed shoots and their shoot lengths. However, there were some differences regarding fresh and dry weights. On the other hand, increased salinity concentrations reduced the number of the newly formed shoots, as well as, their shoot lengths. In addition, liquid media produced more shoots compared with the three solidifying agents used. However, there were significant differences among the concentrations of solidifying agents used.

 

Key words: Carbon source, salinity, gelling agent, fig (Ficus carica L.).