African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Antioxidant activities and GC-MS profiling of fractions of methanol extract of Andrographis paniculata

Benjamin Olusola Omiyale
  • Benjamin Olusola Omiyale
  • Department of Medical Biochemistry, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
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Kayode Olayele Karigidi
  • Kayode Olayele Karigidi
  • Nutritional and Industrial Biochemistry Research Laboratories, University of Ibadan, Nigeria.
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  •  Received: 04 January 2023
  •  Accepted: 13 July 2023
  •  Published: 31 August 2023

Abstract

This study was intended to investigate the antioxidant activities of different solvent fractions (hexane (HEX), chloroform (CHL), ethylacetate (ETHYL) and methanol (MET) fractions) of methanol extract of Andrographis paniculata and GC-MS profiling of the most active fraction (MET). The crude methanol extract was fractionated using vacuum liquid chromatography method. Antioxidant activity was evaluated using total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), ferric reducing antioxidant potential (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2-azobis-3-ethylbenzothiazoline-6-sulfonate (ABTS), and Nitric oxide (NO) radical scavenging abilities. GC-MS was conducted to determine phytochemical present in methanol fraction. The results indicated that MET possessed significantly (p < 0.05) higher TPC (33.98 ±1.63 mg GAE/g), TFC (15.81 ± 0.9 5mg QUE/g), TAC (21.44 ± 0.29 mg AAE/g) and FRAP (57.87 ± 0.88 mg Fe2+/g) than the other fractions. Also, MET exhibited the highest scavenging (ABTS, DPPH and NO) abilities of all the fractions. The GC-MS profiling of methanol fraction showed abundance presence of 2, 5- octadecadienoic-methyl ester and hexadecanoic acid-methyl ester. In conclusion, various fractions of A. paniculata have antioxidant abilities and could be used in diseases associated with free radicals.

 

Key words: Andrographis paniculata, antioxidant, solvent-fractions, GC-MS.