African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Screening for attractants compatible with entomopathogenic fungus Metarhizium anisopliae for use in thrips management

David Kupesa Mfuti
  • David Kupesa Mfuti
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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Sevgan Subramanian
  • Sevgan Subramanian
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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Saliou Niassy
  • Saliou Niassy
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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Daisy Salifu
  • Daisy Salifu
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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Hannalene du Plessis
  • Hannalene du Plessis
  • Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa.
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Sunday Ekesi
  • Sunday Ekesi
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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Nguya Kalemba Maniania
  • Nguya Kalemba Maniania
  • International Centre of Insect Physiology and Ecology (icipe), Duduville Campus, P. O. Box 30772-00100, Nairobi, Kenya.
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  •  Received: 04 December 2015
  •  Accepted: 21 March 2016
  •  Published: 27 April 2016

Abstract

Several thrips attractants were screened for compatibility with Metarhizium anisopliae (Metchnikoff) Sorokin (Hypocreales: Clavicipitaceae) and a subset of these for attraction to Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae). Conidial germination and germ tube length of M. anisopliae were used as indicators of its compatibility with thrips attractant. Conidial germination and germ tube length differed significantly according to volatiles of different attractants. The highest conidial germination (76.5±3.5%) and longest germ tube length (130.3±13.4 µm) were recorded in the control, followed by methyl anthranilate (63.8±3.8%; 103.8±8.4 µm), cis-jasmone (61.8±5.9%; 93.8±14.4 µm) and trans-caryophyllene (57.7±6.5%; 96.3±15.5 µm) which were found compatible with M. anisopliae. A Pearson correlation test indicated a significant positive correlation between conidial germination and germ tube length (r =0.6; P<0.0001). The attraction of M. sjostedti to selected thrips attractant also varied significantly among the attractants. Under field conditions, methyl anthranilate was equally attractive to M. sjostedi as Lurem-TR and could be recommended as a thrips attractant that can be combined with M. anisopliae in autoinoculation devices for potential control of M. sjostedti.

Key words: Semiochemicals, conidial germination, germ tube length, Megalurothrips sjostedti, attraction, persistence, field.