African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Molecular marker screening of tomato, (solanum lycopersicum L.) Germplasm for root-knot nematodes (Meloidogyne species) resistance

Y. Danso1*, R. Akromah2 and K. Osei1
  1Crops Research Institute (CSIR), Kumasi, Ghana. 2Department of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Email: [email protected]

  •  Accepted: 30 December 2010
  •  Published: 28 February 2011

Abstract

 

Tomato is one of the crops in which genetic resistance has specially been effective against root-knot nematodes. In this study, molecular screening was done on some tomato germplasm to detect markers for the gene that confers resistance (Mi) with specific primer (Mi23/F//Mi23/R). The cultivars; VFNT, FLA 505-BL 1172, 2641A, “Adwoa Deede” and Terminator FI showed the marker for the homozygous resistant genotypes (Mi/Mi). The cultivars, Tima and 2644A showed both markers, corresponding to heterozygous resistant genotypes (Mi/mi). Twenty one (21) of the cultivars did not show any of the markers presumably due to non-specificity at the primer-binding sites. Five (5) heterozygous individuals were determined out of 6 resistant cultivars following the Hardy-Weinberg principle in population genetics.

 

Keywords: Germplasm, Meloidogyne incognita, molecular marker, resistance,Solanum lycopersicum.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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