Full Length Research Paper
Abstract
The enzymatic hydrolysis of cassava starch (Mannihot esculenta) to glucose wasstudied; considering various process parameters such as temperature, pH, inoculums age, cell loading and substrate concentration. Aspergillus niger was isolated from deteriorated groundnut seeds and used as amylase source. After characterization, amylase exhibited optimum activity at pH 4.7 and temperature 58°C. The cells appear most viable on the 5th day. An initial rate method was employed in evaluating the kinetic parameters using Michealis-Menten model. The Km and rmaxwas found to be 0.2248 mmol/l and 2.055*10-4 mmol/l.min, respectively. Cassava starch was selected for the study because of its relative abundance in Nigeria compared to other starchy materials.
Key words: Aspergillus niger, amylase, enzymatic hydrolysis, glucose syrup, cassava starch.
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