Abstract

In this article, we investigated self-compatibility index (SCI) in broccoli double haploid (DH) lines, and the relationship and genetic diversity of 15 self-incompatible (SI) broccoli DH lines were analyzed by sequence related amplified polymorphism (SRAP). 11 primer combinations selected from 88 primer pairs revealed a total number of 129 unambiguous bands, 61 of which were polymorphic with a polymorphism frequency of 47.3%. Analyzed by NTSYS software, the genetic similarity coefficient of the 15 broccoli resources ranged from 0.76 to 0.98. Based on the coefficient value of 0.79, these broccoli DH lines were clustered into three multiple-member groups by unweighted pair group method with arithmetic mean (UPGMA) analysis, which provided molecular reference for parent selection in broccoli breeding.

Key words: Brassica oleracea L. var. italica, self-compatibility index, double haploid, genetic diversity, sequence related amplified polymorphism (SRAP).

Abbreviation

SCI, Self-compatibility index; DH, double haploid; SRAP, sequence related amplified polymorphism; UPGMA, unweighted pair group method with arithmetic mean; SI, self-incompatible; SSI, sporophytic self-incompatibility; SRK, S receptor kinase; SP11/SCR, small cysteine-rich secreted protein; DNA,deoxyribonucleic acid; CTAB, cetyl trimethylammonium bromide; PCR, polymerase chain reaction; SC, self-compatible; ORPs, open reading frames; AFLP, amplified fragment length polymorphism.