African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA®

Luis Gonzaga Gutiérrez
  • Luis Gonzaga Gutiérrez
  • Grupo de Investigación en Biodiversidad y Biotecnología, Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira, Carrera 27 #10-02 Barrio Alamos, Pereira, Colombia.
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Rodolfo Lopez-Franco
  • Rodolfo Lopez-Franco
  • Programa de Biología, Laboratorio de Biotecnología, Centro de Estudios e Investigaciones en Biodiversidad y Biotecnología (CIBUQ), Universidad de Quindío, Carrera 15 Calle 12 Norte, Armenia, Colombia.
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Tito Morales-Pinzón
  • Tito Morales-Pinzón
  • Grupo de Investigación en Gestión Ambiental Territorial, Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira, Carrera 27 #10-02 Barrio Alamos, Pereira, Colombia.
  • Google Scholar


  •  Received: 09 April 2016
  •  Accepted: 27 June 2016
  •  Published: 13 July 2016

Abstract

The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semi-solid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and use of semi-solid culture medium, which consisted of MS supplemented with 3.0 mg L-1 of the cytokinin benzylaminopurine (BAP). Equipment consisted of 20 vessels for automated RITA®, each containing 200 ml of culture medium. Immersions were performed for 2 min at two different frequency intervals (6 and 8 h). Large clumps of G. angustifolia with 1, 2 or 3 stems were inoculated depending on the treatment. Best results were obtained with four immersion cycles per day (every 6 h), with a multiplication index of 2.7 shoots per original explant (axillary buds) and greater rhizome growth. Overall, the temporary immersion system performed better than the semi-solid medium in terms of shoot multiplication rates and rhizome growth. Further studies should be conducted to develop an application for RITA® for use in the commercial production of G. angustifolia.

Key words: Giant bamboo, temporary immersion system RITA, rhizome.

Abbreviation

BAP, 6-Benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid; GA, gibberellic acid; IAA, indoleacetic acid; MS, Murashige and Skoog (1962); NAA, naphthalene acetic acid.