African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

Bornali Gohain1*#, Tirthankar Bandyopadhyay1#, Priyadarshini Bhorali2, Sangeeta Borchetia3, Raju Bharalee1, Sushmita Gupta1, Niraj Agarwala1, Neelakshi Bhattacharyya1, Ranjit Singh1, Prasenjit Bhagawati1, Parveen Ahmed1, M.C.Kalita4 and Sudripta Das1
1Department of Biotechnology, Tea Research Association, Tocklai Experimental Station, Jorhat-785008, Assam, India.  2Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat- 785013, Assam, India. 3Department of Zoology, JB College, Jorhat-785001, Assam, India. 4Department of Biotechnology, Gauhati University, Guwahati-781014, Assam, India.   #These authors contributed equally to this work.
Email: [email protected]

  •  Accepted: 24 August 2011
  •  Published: 26 June 2012

Abstract

The present experiment is an effort to find a stable reference gene in Camellia sinensisand Camellia assamica under different biotic and abiotic stresses. This study evaluatethe variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubisco-bis-phosphatase oxygenase (RuBP) andCamellia tubulin (CaT) as reference genes were validated by geNorm and BestKeeper programs. The finding reveals 18S rRNA and RuBP to be the most stably expressed housekeeping genes, the latter being the first report of its kind in tea. The finding paves the way for their application in accurate quantification of trait specific gene expression and other genomic studies in tea.

 

Key words: Camellia sinensis, Camellia assamica, qPCR, BestKeeper, geNorm, housekeeping gene.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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