African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Type I collagen from bullfrog (Rana catesbeiana) fallopian tube

Tzuching Wang1, Shenchung Lin2, Yingpei Shen1,3, Shangyu Liu2, Tzuling Wang-McCall2, Menghsiung Chin2, Tachen Lin5, Clinton Yang6, Winhui Wu7 and Chiching Yang7,*
1Department of Food and Beverage Management Section, Tzuhui Institute of Technology, Pingtung, 92641, Taiwan, Republic of China. 2Department of Hospitality Management, Tajen University, Pingtung, 90741, Taiwan, Republic of China. 3Ph.D. Program in Management, Dayeh University, Changhua 51591, Taiwan, Republic of China. 4Department of Tourism and Leisure Management, Fortune Institute of Technology, Kaohsiung City 83160, Taiwan, Republic of China. 5Department of Hospitality Management, Meiho University, Pingtung, 91202, Taiwan, Republic of China. 6Department of Pharmaceutical Sciences, Albany College of Pharmacy, Albany, New York, 12208-3492, USA. 7Department of Food Science and Technology, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, Republic of China
Email: [email protected]

  •  Accepted: 11 March 2011
  •  Published: 08 August 2011

Abstract

Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog (Rana catesbeiana) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components (α1 and α2) and was classified as type I collagen. PSC was found to contain 19.5% of amino acids. PSC exhibited a maximum absorbance at 230 nm, but little absorbance near to 280 nm. The denaturation temperature for PSC was determined to be 29.6°C. These results suggest that bullfrog fallopian tube has potential as a supplement to the sources of vertebrate collagens.

 

Key words: Bullfrog (Rana catesbeiana), pepsin-soluble collagen, type I collagen.

Abbreviation

 PSC, Pepsin-soluble collagen; SDS-PAGE, sodium dodecyl sulphate polyacylamide-gel electrophoresis; CBB, coomassie brilliant blue R-250;FTIR, fourier transform infrared spectroscopy.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 42
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES