African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular characterization of Azotobacter spp. nifH gene Isolated from marine source

Rajeswari K.1* and Mangai Kasthuri G.2
  1G. R. Damodaran College of Arts and Science, Coimbatore, India. 2Madurai Meenakshi College, Madurai, India.
Email: [email protected]

  •  Accepted: 01 October 2009
  •  Published: 15 December 2009

Abstract

 

The nifH gene sequence of the nitrogen-fixing bacterium Azotobacter spp. was determined with the use of polymerase chain reaction (PCR). The Azotobacterspecies was isolated from marine source in two different seasons. They were cultivated under laboratory conditions using Nitrogen free Azotobacter specific medium. We observed that they were present in both seasons. The phylogenetic tree revealed that our isolated Azotobacter spp. was distantly related to uncultivated and uncultured organisms. They did not form any branch with other Azotobacterspp. in the data base.

 

Key words: Azotobacter, nifH, PCR, nitrogenase, biological nitrogen fixation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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