African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Bacterial adherence: the role of serum and wound fluid

Yah, S. C.1*, Haruna, T.2, Enabulele, I. O.2 and Yusuf E. O.2
  1Department of Biological Sciences, College of Science and Technology, Covenant University, Km 10 Idiroko Road, PMB 1023 Ota, Ogun State, Nigeria. 2Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Edo State, Nigeria.
Email: [email protected]

  •  Accepted: 24 July 2008
  •  Published: 05 November 2008

Abstract

 

Bacteria are known to initiate wound infections and have been found associated with wound infections.  The purpose of this study was to investigate the bacterial adherence potential (BAP) wounds. The bacteria used were Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Staphylococcus aureus isolated from infected wounds and assayed for their adherence ability using wound fluid and serum. The BAP were achieved by exposing the pathogens to freshly excised wounds. The adhered bacteria were then eluded and quantified using log (CFU/cm2) on Mueller Hinton Agar per cm2 of tissue. The results indicated that wound fluid and serum has a remarkable bacterial adherence potential (BAP) when exposed to freshly injured wounds as when compared to distilled water and no agent.  

 

Key words: Bacteria wound, adherence, wound fluid, serum.