African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Perforate on CHO cell membranes induced by electromagnetic pulses irradiation observed by atomic force microscopy

Lihua Zeng1, Changxu Zou2, Jie Zhang1, Xiaowu Wang1, Xia Miao1, Dongqing Ren1, Yurong Li1 and Guozhen Guo1*
1Department of Radiation Medicine Faculty of Preventive Medicine, Fourth Military Medical University, Changle west road 17, Xi’an 710032, China. 2Anatomy Department and K. K. Leung Brain Research Center, Fourth Military Medical University, Changle west road 17, Xi’an 710032, China.
Email: [email protected], [email protected]

  •  Accepted: 16 June 2009
  •  Published: 17 June 2009

Abstract

Atomic force microscopy (AFM) has been used to visualize the morphological change on the surface of Chinese hamster ovary (CHO) cell membranes before and after electromagnetic pulses (EMP) irradiation. The results show that there were different sizes and shapes of membrane perforate (width ranging from 0.39 - 0.66 μm; depth of 392.95 nm) after 200 pulses of EMP irradiation at 400 kV/m. The results suggested that EMP irradiation can directly cause CHO cell membranes to perforate. The numbers of cell membrane perforates increased with an increase in applied EMP field intensity and an increase in the number of EMP pulses. EMP induced membrane perforate may play a very important role in EMP biological effects.

Key words: Electromagnetic pulse (EMP), atomic force microscope, CHO cell, cell membrane.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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