African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12412

Full Length Research Paper

Genetic diversity and population structure of sweet cassava using simple sequence repeat (SSR) molecular markers

Tiago Ribeiro da Costa1, Pedro Soares Vidigal Filho1*, Maria Celeste Gonçalves-Vidigal1, Marta Zulema Galván2, Giselly Figueiredo Lacanallo1, Luciano Ivano da Silva1 and Marcus Vinicius Kvitschal3
  1Departamento de Agronomia da Universidade Estadual de Maringá: Av. Colombo, n. 5790, CEP 87020-570, Maringá, Paraná, Brasil. 2Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Fisiología Vegetal - CONICET, La Plata, Argentina. 3Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri / Estação Experimental de Caçador: Rua Abílio Franco, nº 1500, C.P. 591, CEP 89500-000, Caçador, Santa Catarina, Brasil.
Email: [email protected]

  •  Accepted: 01 February 2012
  •  Published: 31 March 2013



The objective of this study was to evaluate the population structure and genetic diversity among 66 sweet cassava (Manihot esculenta Crantz) traditional accessions collected in Maringa, Parana, Brazil, using microsatellite molecular markers. Population structure was analyzed by means of genetic distances and probabilistic models; allelic frequencies were used in order to assess the genetic diversity indexes (Ht, Ho, PIC, % polymorphism and number of alleles) for each locus studied. All evaluated loci were polymorphic and the average was highly heterozygote. The number of alleles per locus was low, suggesting that restrict genetic base is a consequence of accession exchange and a reduced number of ancestors in the population. The polymorphic information content (PIC) values showed considerable genetic diversity with a mean value of 0.5076 and peak of 0.5707 for locus GA140. The microsatellites markers revealed a wide genetic variability among the traditional accessions evaluated. Moreover, the sweet cassava populations were separated in two groups using two analytical methods (probabilistic and genetic distances). The most divergent accessions were BGM 17, BGM 20, BGM 51 and BGM 95. On the other hand, the most similar accessions were BGM 25, BGM 33, BGM 37, BGM 59 and BGM 214. Hybrid combinations formed by the most divergent combinations, especially between BGM 51 × BGM 296, BGM 95 × BGM 222 and BGM 20 × BGM 12, are the most promising ones for future sweet cassava breeding programs.


Key words: Genetic diversity, Manihot esculenta Crantz, microsatellite markers.