African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic variability in Asparagus racemosus (Willd.) from Madhya Pradesh, India by random amplified polymorphic DNA

  Neetu Vijay1*, Pramod Sairkar1, Nipun Silawat1, R. K. Garg1 and N. N. Mehrotra1,2      
  1Centre of Excellence in Biotechnology, M. P. Council of Science and Technology Vigyan Bhawan, Nehru Nagar, Bhopal-462003, Madhya Pradesh, India. 2Central Drug Research Institute, Lucknow-226001, India.
Email: [email protected]

  •  Accepted: 03 April 2009
  •  Published: 20 July 2009

Abstract

 

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Asparagus racemosus (Willd.) an important medicinal plant collected from 7 different locations covering Madhya Pradesh. High level of genetic similarity was observed in the collected accessions. 4 random primers generated a total 39 polymorphic bands out of the total 71 bands. A low genetic diversity among the A. racemosus samples was revealed by RAPD analysis. Cluster analysis based on dice coefficient showed 2 major groups. This distributive pattern of genetic variation of A. racemosus accessions provides important baseline data for conservation and collection strategies for this species.

 

Key words: Genetic diversity, Asparagus racemosus, RAPD, polymorphism.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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