Abstract

This communication provides an insight into the emerging of new infection “red eye” in Pangasius sutchi and aimed to screen the prime pathogens involved in disease. The pathogen was isolated from diseased P. sutchi and characterized by morphological, biochemical and molecular approach, which includes 16s r RNA gene sequencing. Polymerase chain reaction (PCR) amplified 16s RNA was separated using agarose gel electrophoresis, eluted product was sequenced and BLAST analysis was carried out to identify the pathogens. Identified virulent bacterial strain Acinetobacter schindleri with LD₅₀ 10^8.35 initiated re-infection in experimentally in infected Pangasius fingerlings. This study provided the evidence of A. schindleri which is true causative agents in red eye disease in P. sutchi. To the best of knowledge of this study, there was no track record of A. schindleri eye infection in fishes till date around the globe.

Key words: Pangasius sutchi, 16s r- RNA gene sequencing, Acinetobacter schindleri, LD₅₀.

Abbreviation

Abbreviations: FCR, Food conversion rates; MSY, maximum sustainable yields; PCR, polymerase chain reaction; SDS, sodium dodecyl sulfate; CTAB, cetyl trimethyl ammonium bromide; BOD, biological oxygen demand; COD, chemical oxygen demand.