African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Assessment of the genetic diversity conservation in three tall coconut (Cocos nucifera L.) accessions regenerated by controlled pollination, using microsatellite markers

YAO Saraka Didier Martial1,2,4, KONAN Konan Jean Louis2*, POKOU N’Da Désiré1, KONAN Kouamé Jean Noel1, ISSALI Auguste Emmanuel2, SIE Raoul Sylvère3 and ZORO Bi Irié Arsène4
1Laboratoire Central de Biotechnologies, Centre National de Recherche Agronomique, Km 17 Route Dabou, 01 BP 1740 Abidjan 01, Côte d'Ivoire. 2Laboratoire de Fécondation Artificielle, Centre National de Recherche Agronomique, Station Marc Delorme de Port Bouet, 07 BP 13 Abidjan 07, Côte d'Ivoire. 3Laboratoire de Biologie et d’Amélioration des Productions Végétales, UFR Sciences de la Nature, University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d'Ivoire. 4Laboratoire de Génomique Fonctionnelle et Amélioration Génétique, UFR Sciences de la Nature, University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d'Ivoire.  
Email: [email protected], [email protected]

  •  Accepted: 12 April 2012
  •  Published: 15 May 2013

Abstract

Controlled pollination process is used during the regeneration of ageing accessions in the International Coconut Genebank for Africa and Indian Ocean (ICG-AIO). The effectiveness of this process has not yet been evaluated. This study aimed to evaluate the effectiveness of controlled pollination method by investigating the level of molecular resemblance between the regenerated (G1) and parental (G0) populations of three tall coconut accessions, Mozambique Tall (MZT), Gazelle Peninsula Tall(GPT) and Tahitian tall (THT) using 15 microsatellites (SSR) markers. The results indicate a relative reduction of gene diversity during regeneration. It decreased from 0.690 to 0.587, but, low values of Jaccard’s dissimilarity index were found between regenerated and parental populations of tall coconut, varying from 0.072 to 0.133. Low values of genetic diversity between G0 and G1 tall coconut accessions (DST), ranging from 0.005 to 0.007, were recorded. Consequently, using controlled pollination technical as regeneration method of the genebank is effective for satisfying maintenance of the genetic integrity of the original coconut accessions.

 

Key words: Tall coconut palm, controlled pollination, regenerated accessions, genetic diversity, microsatellites markers.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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