Full Length Research Paper
Abstract
Two Escherichia coli isolates which were isolated from the urine of patients in state hospitals in KwaZulu-Natal, South Africa were investigated to determine the sequence of the TEM β-lactamases responsible for their resistance to β-lactamase inhibitors. The isolates were subjected to MIC determinations, iso-electric focusing analysis, plasmid analysis, polymerase chain reaction (PCR) for the detection of β-lactamase genes and sequencing of the blaTEM. Analysis of the nucleotide sequences revealed the presence of two novel TEM β-lactamases, TEM-145 and TEM-146 which had the R244H mutation. Mutations at position 244 have been previously reported in other inhibitor-resistant TEMs (IRTs).
Key words: Escherichia coli, plasmid-mediated, TEM β-lactamase.
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