African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Biolistic inoculation of cassava (Manihot esculenta Crantz) with South African cassava mosaic virus

M. Makwarela
  • M. Makwarela
  • School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa, International Laboratory for Tropical Agricultural Biotechnology (ILTAB), Danforth Plant Science Center, 975 Warson Road, St Louis, MO 63132, Missouri, U.S.A.
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N.J. Taylor
  • N.J. Taylor
  • International Laboratory for Tropical Agricultural Biotechnology (ILTAB), Danforth Plant Science Center, 975 Warson Road, St Louis, MO 63132, Missouri, U.S.A.
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C.M. Faquet2
  • C.M. Faquet2
  • International Laboratory for Tropical Agricultural Biotechnology (ILTAB), Danforth Plant Science Center, 975 Warson Road, St Louis, MO 63132, Missouri, U.S.A.
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M.E.C. Rey
  • M.E.C. Rey
  • School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa.
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  •  Accepted: 24 October 2005
  •  Published: 16 January 2006

Abstract

Cassava mosaic disease (CMD) is undoubtedly the most widespread disease in cassava (Manihot esculenta Crantz) in sub-Saharan Africa. While African cassava mosaic virus-Kenya (ACMV-KE), African cassava mosaic virus-Uganda (ACMV-UG), African cassava mosaic virus-India (ACMV-IC), East African cassava mosaic virus-Cameroon (EACMV-CM) and a recombinant East African cassava mosaic virus-India/Cameroon (EACMV-CM/IC) can be transmitted easily and repeatedly toNicotiana benthamiana plants, difficulty in mechanical transmission and multiplication of EACMV-UG and South African cassava mosaic virus (SACMV) in N.benthamiana appears to place them biologically in a different group. In vitro grown 3-week-old cassava plants were biolistically inoculated with SACMV DNA A and Bdimers and infectivity measured using a system based on visual assessment. We report for the first time successful infection, induced by SACMV DNA A and B dimer, of cassava cultivar TMS60444 using biolistic inoculation. Typical mosaic symptoms started to show at 14 days post-inoculation (dpi) in infected cassava plants, and SACMV replication was confirmed using PCR. Potential applications of biolistic infection of SACMV are also discussed.

 

Key words: Cassava, biolistic, inoculation, SACMV.

Abbreviation

CMD, Cassava mosaic disease; ACMV, African cassava mosaic virus; EACMV, East African cassava mosaic virus; ACMV-KE, African cassava mosaic virus-Kenya; ACMV-UG, African cassava mosaic virus-Uganda; ACMV-IC, African cassava mosaic virus-India; EACMV-CM, East African cassava mosaic virus-Cameroon; EACMV-CM/IC, East African cassava mosaic virus-India/Cameroon;  SACMV, South African cassava mosaic virus; dpi, days post-inoculation; PCR, polymerase chain   reaction;  IR,  intergenic  region;   CR,   common   region.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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