African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Molecular identification of Ornithobacterium rhinotracheale in turkeys in Isfahan province of Iran

Abbas Doosti1*, Ali Sharifzadeh2, Hamed Ghasemi1 and Javad Vaez1
1Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. 2Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Email: [email protected]

  •  Accepted: 01 June 2011
  •  Published: 01 August 2011

Abstract

Ornithobacterium rhinotracheale (ORT) is a gram negative, pleomorphic, rod shaped and non-motile bacterial pathogen mostly known to cause respiratory tract infections in turkeys. Ornithobacteriosis is an infectious disease of avian species that has been reported in almost all countries around the world. The objective of this study was to determine the prevalence of ORT in turkeys in Isfahan province of Iran. DNA was extracted from 375 collected tracheal swabs and lungs samples and amplified by ORT 16S rRNA gene specific primers using the PCR technique. ORTDNA was detected in 75 samples (19.93%) of broiler turkeys in Isfahan province of Iran. The results of this study demonstrated the widespread of ORT in broiler turkeys and confirmed that infection with ORT have a high prevalence in Iran.

 

Key words: Ornithobacterium rhinotracheale (ORT), turkey, polymerase chain reaction, Iran.

Abbreviation

ORT, Ornithobacterium rhinotracheale

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 40
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES