African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Lipid peroxide, glutathione and glutathione-dependent enzyme (GST) in mixed zooplankton from the North-West Coast of India: Implication for the use of environmental monitoring

Vijayakumar Rathod* and Patil Balkrishna A.
National Institute of Oceanography (CSIR) Dona Paula, Goa-403004, India.
Email: [email protected]

  •  Accepted: 21 March 2011
  •  Published: 01 August 2011

Abstract

This work deals with an experiment on mixed zooplanktonic organisms collected from the shore water of Diu coast. The study analyzed and measured lipid peroxidation (LPX), as a marker of oxidative stress and glutathione-s-transferase (GST) activity, as a marker of organic pollution. Both LPX and GST activities were highest at the stations close to the shore and marginally decreased along with transect. The reduced glutathione (GSH) levels were highly variable. The results were discussed in relation to the biomarker application of mixed zooplankton, lipid peroxidation (LPX), glutathione-S-transferase (GST) and reduced glutathione (GSH). The results indicated that, the three potential markers (LPX, GST and GSH), could be used as a measure for bio-monitoring the costal ecosystem using mixed zooplankton as suitable organisms.

 

Key words: Zooplankton, lipid peroxidation, glutathione-S-transferase, reduced glutathione.

Abbreviation

LPX, Lipid peroxidation; GST, glutathione-S-transferase; GSH, reduced glutathione; ROS, reactive oxygen species; SOD, superoxide dismutase;GPx’s,    peroxidations; SH, cystin, TBA, thiobarbituric acid; SDS; sodium dodecyl sulfate; TBARS, thiobarbituric acid reactive substances; BHT, butylated hydroxyl toluene; PAH, petroleum aromatic hydrocarbon; DPPH, 2-2-Diphenyl-1-picrylhydrazyl; CDNB, 1-chloro-2, 4-dinitrobenzene.