African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12291

Full Length Research Paper

Effect of methanolic extract of Dennettia tripetala (pepper fruit) on biomarkers of oxidative stress and lipid peroxidation in type 2 diabetic male wistar rats

Nwaeme Ogochukwu
  • Nwaeme Ogochukwu
  • Department of Human Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ude Victor Chibueze
  • Ude Victor Chibueze
  • Department of Medical Biochemistry, College of Medicine, Enugu State University of Science & Technology, Parklane Enugu Nigeria.
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Augustine Ndubuisi
  • Augustine Ndubuisi
  • Department of Medical Biochemistry, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ugwudike Patrick
  • Ugwudike Patrick
  • Department of Medical Biochemistry, College of Medicine, Enugu State University of Science & Technology, Parklane Enugu Nigeria.
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Agu Francis
  • Agu Francis
  • Department of Human Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Celestine Ani
  • Celestine Ani
  • Department of Human Physiology, Faculty of Basic Medical Sciences, Enugu State University of Science & Technology, Parklane Enugu Nigeria.
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Emeka Ugwuishi
  • Emeka Ugwuishi
  • Department of Human Physiology, Faculty of Basic Medical Sciences, Enugu State University of Science & Technology, Parklane Enugu Nigeria.
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Sunday Njoba
  • Sunday Njoba
  • Department of Human Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Lilian Ndubuisi
  • Lilian Ndubuisi
  • Department of Human Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Hector Obianyido
  • Hector Obianyido
  • Department of Medical Biochemistry, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Joachin Ezeokeke
  • Joachin Ezeokeke
  • Department of Medical Biochemistry, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Nwachukwu Daniel
  • Nwachukwu Daniel
  • Department of Human Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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  •  Received: 15 December 2019
  •  Accepted: 15 January 2020
  •  Published: 29 February 2020

Abstract

Oxidative stress resulting from chronic hyperglycemia in diabetes is due to high production of reactive oxygen species and/or a decrease in the antioxidant defense system activity. The study was designed to investigate the ameliorative effect of methanolic extract of Dennettia tripetala (DT) on oxidative stress and blood glucose level in type II diabetic male Wistar rats. Type II diabetes was induced by a single intraperitoneal injection of streptozotocin (40 mg/kg) after 2 weeks of 10% fructose diet. Twenty-five (25) rats were randomly divided into five groups, namely: 1 (normal control), 2 (negative control), 3 (10 mg/kg of glibenclamide), 4 (100 mg/kg of DT extract) and 5 (200 mg/kg of DT extract). The administration of the extract caused a significant decrease in blood glucose levels in all treatment groups. A significant (P<0.01) decrease in malonaldehyde (MDA) activity was observed in the group treated with 100 and 200 mg/kg of DT extract compared to diabetic control. Catalase (CAT) activity showed a significant (P<0.05) increase in the group treated with 200 mg/kg of DT extract compared to the normal control. However, the extract did not affect GSH-Px, SOD and CAT activities. The findings suggest ameliorative effect of DT extract in diabetic Wistar rats.

Key words: Dennettia tripetala, diabetes mellitus, oxidative stress, lipid peroxidation, Wistar rats.