African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Biological evaluation of 32 different essential oils against Acidovorax citrulli, with a focus on Cinnamomum verum essential oil

Okhee Choi#
  • Okhee Choi#
  • Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Search for this author on:
  • Google Scholar
Su Kyung Cho#
  • Su Kyung Cho#
  • Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea.
  • Search for this author on:
  • Google Scholar
Jinwoo Kim*
  • Jinwoo Kim*
  • Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea.
  • Search for this author on:
  • Google Scholar


  •  Received: 15 October 2015
  •  Accepted: 13 January 2016
  •  Published: 27 January 2016

Abstract

Bacterial fruit blotch (BFB) of watermelon caused by Acidovorax citrulli (ACC) is one of the most severe diseases of watermelon worldwide. Antibacterial activity of 32 essential oils (EOs) was evaluated against ACC using disk-diffusion assays. The oil from cinnamon exhibited the greatest antibacterial activity. Using gas chromatography-mass spectrometry (GC-MS), the major components of cinnamon oil were analyzed. Among the various components of cinnamon oil, benzaldehyde and cinnamaldehyde exhibited the effective antibacterial activities against ACC. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of benzaldehyde and cinnamaldehyde were measured using broth dilution assays. The MICs against ACC of benzaldehyde and cinnamaldehyde were 0.1 and 0.01% (v/v), respectively. The MBCs of benzaldehyde and cinnamaldehyde against ACC were 0.2 and 0.02% (v/v), respectively. Also, 0.2% (v/v) levels of cinnamon oil, benzaldehyde and cinnamaldehyde completely killed ACC cells artificially contaminating watermelon seeds. This study suggests that cinnamon oil and its bioactive components, benzaldehyde and cinnamaldehyde, have potential for application as natural agents for the prevention and treatment of BFB.

Key words: Acidovorax citrulli, bacterial fruit blotch, cinnamon oil, essential oil.

#This authors contributed equally to this work

Abbreviation

BFB, Bacterial fruit blotch; ACC, Acidovorax citrulli; EO, essential oil; GC-MS, gas chromatography-mass spectrometry; MIC, minimum inhibitory concentration; MBC, minimum bactericidal concentration.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 15 No. 4
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES