African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Molecular characterization and phylogenetic relationships among and within species of Phalaenopsis (Epidendroideae: Orchidaceae) based on RAPD analysis

  A. Niknejad1*, M. A. Kadir1, S. B. Kadzimin2, N. A. P. Abdullah2 and K. Sorkheh3
  1Department of Agriculture Technology, Faculty of Agriculture, University Putra, Malaysia. 2Department of Crop Science, Faculty of Agriculture, University Putra, Malaysia. 3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahr-e-Kord   University, Iran.
Email: [email protected]

  •  Accepted: 31 August 2009
  •  Published: 19 October 2009

Abstract

 

Random amplified polymorphic DNA (RAPD) analysis for 20 species ofPhalaenopsis was conducted to determine their genetic distances and relationships. Among 20 different primers used for RAPD analysis, 10 primers showed polymorphism, and according to the primer type, 26 to 54 DNA fragments were amplified. A total of 414 polymorphic fragments were generated by 10 primers and used for correlation group analysis. The highest value of Similarity index was 0.28 between Ph. violacea malaysia and Ph. violacea witte. The dendrogram resulting from UPGMA (Unweighted Pair Group Method using Arithmetic average) hierarchical cluster analysis separated the original species into three groups: The first group had five species of Ph. violacea bluePh. belinaPh. violacea  malaysia,Ph. violacea witte, and Ph. gigantea; the second group included Ph. lamelligera, Ph. amabilis, Ph. parishii, Ph. labbi nepal, Ph. speciosa, Ph. lobbi yellow, Ph. venosa, Ph. hieroglyphica, and Ph. maculata; the third group consisted of Ph. minho princess, Ph. leopard prince, Ph. mannii, Ph. modesta, Ph. cornucervi and Ph. pantherina. RAPD markers can thus be successfully applied in this economically important group of orchids for the study of molecular characterization and relationships. The data acquired from this study could be used for identification and classification of other orchid genera and oriental Phalaenopsis.

 

Key words: Phalaenopsis, Random Amplified Polymorphic DNA (RAPD), classification, molecular characterization.