African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Analysis of sarcoplamic proteins in natural populations of mountain trout (Salmo trutta macrostigma Dumeril, 1858) with SDS-PAGE

Orhan Demir1, Ali Günlü*1, Fahrettin Kucuk1, Ä°skender Gulle2 and Erkan Gumus3
  1Fisheries Faculty, Süleyman Demirel University, EÄŸirdir-Isparta, Türkiye. 2Science and Arts Faculty, Mehmet Akif Ersoy University, Burdur, Türkiye. 3Fisheries Faculty, Akdeniz University, 07058, Antalya, Türkiye.
Email: [email protected]

  •  Accepted: 08 August 2011
  •  Published: 21 September 2011

Abstract

 

Molecular weights and band counts of sarcoplasmic proteins in four populations of Salmo trutta macrostigma living in Mediterranean region of Turkey were analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method and densitometric analysis. 13, 17, 14 and 15 sarcoplasmic protein bands were obtained, respectively from EÅŸen Stream (I), Aksu Stream (II), Alara Stream (III) and Alakır Stream (IV) populations. Protein bands with molecular weights 210, 93 and 30 kDa were found solely in population II. 116 kDa bands were present in all populations but population I with density of bands with molecular weights over 97 kDa was smaller than all the other populations. Densitometric analysis of population IV showed that it differs from the three other poulations by having all protein bands except for 45 kDa band which were at low densities.

 

Key words: Sarcoplazmic proteins, mountain trout, Salmo trutta macrostigma, SDS-PAGE, electrophoresis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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