African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)

Yanping Hu1,2, Xiaolong Xie1,2, Li Wang1, Jian Yang1,2, Huaigang Zhang1* and Yi Li1*
  1Key Laboratory of Adaptation and Evolution of Plateau Biota，Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P. R. China. 2Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Email: [email protected], [email protected]

  •  Accepted: 26 March 2009
  •  Published: 17 June 2009

Abstract

 

Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A260/A280 absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.

 

Key words: Rhubarb, Rheum tanguticum, DNA extraction, ISSR, secondary metabolites.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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