African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Production, purification and characterization of two recombinant DNA-derived N-terminal ovine growth hormone variants: oGH3 and oGH5

Amtul Jamil Sami1* O. C. Wallis2 and M. Wallis2
  1Institute of Biochemistry and Biotechnology, University of the Punjab Lahore, Pakistan. 2Biochemistry Department, School of Life Sciences, University of Sussex, Brighton BN1 9QG England, UK.
Email: [email protected]

  •  Accepted: 15 February 2008
  •  Published: 17 June 2008

Abstract

 

Two recombinant DNA-derived variants of ovine growth hormone were produced, purified, characterized and compared with the authentic pituitary derived GH. The variants oGH3 and oGH5 were isolated by differential centrifugation method and were purified after refolding by ion-exchange chromatography and gel filtration. Both the proteins showed single band on SDS-PAGE and had molecular weight and iso-electric point closer to authentic pituitary GH. The variants oGH3 and oGH5 were compared with the authentic pituitary derived GH in radio immuno assays, radio receptor assays and binding with the monoclonal antibodies OA 11 and OA12. 

 

Key words: Growth hormone, GH, oGH3, oGH5, radioimmuno assay, radio receptor assay.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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