This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and 6.56±3.06 µg/g in R. tigrina captured from non-industrial site. From the results of the present study, it may be concluded that frogs are good bio-indicator species to estimate the level of heavy metals pollution of various water sources in some countries.
Key words: Copper, bio-indicator, atomic absorption spectrometry (AAS), aquatic habitat.
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