African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

Muhammad Nasir
  • Muhammad Nasir
  • Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
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Tariq Mahmood Ansari
  • Tariq Mahmood Ansari
  • Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
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Hira Javed
  • Hira Javed
  • Department of Environmental Sciences Forman Christian College University, Lahore, Pakistan.
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Ghulam Yasin
  • Ghulam Yasin
  • Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
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Aleem Ahmad Khan
  • Aleem Ahmad Khan
  • Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800, Pakistan.
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Muhammad Shoaib
  • Muhammad Shoaib
  • Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
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  •  Received: 29 March 2017
  •  Accepted: 16 March 2018
  •  Published: 29 February 2020

Abstract

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and 6.56±3.06 µg/g in R. tigrina captured from non-industrial site. From the results of the present study, it may be concluded that frogs are good bio-indicator species to estimate the level of heavy metals pollution of various water sources in some countries.

 

Key words: Copper, bio-indicator, atomic absorption spectrometry (AAS), aquatic habitat.