African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12465

Full Length Research Paper

Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect

Kumar Suranjit Prasad1*, Darshit Pathak1, Ankita Patel1, Palak Dalwadi1, Ram Prasad2, Pradip Patel1 and Kaliaperumal Selvaraj3
1Department of Environmental Biotechnology, Ashok & Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh vidyanagar, Anand, Gujarat, 388121, India. 2Amity institute of microbial technology, Amity University, Sector 125, Noida, U.P., 201303, India. 3Nano and Computational Materials Lab, Catalysis Division, National Chemical Laboratory, Council of Scientific and Industrial Research, Pune - 411008, India.
Email: [email protected]

  •  Accepted: 06 June 2011
  •  Published: 03 August 2011


A green synthesis of silver nanoparticle was carried out using tobacco leaf extract.  Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, TEM, EDAX, FT-IR and photoluminescence study, respectively. UV-Vis absorption spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. Excitation maximum and emission maximum obtained from photoluminescence study were found at 414 and 576 nm, respectively. TEM analysis showed average particle size of 8 nm, while SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis indicated the involvement of carboxyl (-C= O), hydroxyl (-OH) and amine (-NH) functional groups of tobacco leaf extract in preparation of silver nanoparticles. EDAX analysis showed proportion of silver (54.55%) among other elements in nanoparticle. Pseudomonas aeruginosa and Escherichia coli DH5α showed highest sensitivity towards silver nanoparticles.


Key words: Silver nanoparticles, tobacco, UV-Vis absorption, photoluminescence, FT-IR spectroscopy.