African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Screening of thermophilic neutral lipase-producing Pseudomonas sp. ZBC1 and improving its enzymatic activity

Cheng Xing1,2,3, Pu You-guang1,2,3, Zhu Chen-xi1,2,3, Ren Zhi-lin2, Jiang Ming-xing2, Chen Yan1,2,3 and Zhang Bu-chang1,2,3*
  1Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China. 2Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China. 3School of Life Sciences, Anhui University, Hefei, Anhui 230601, China.
Email: [email protected]

  •  Accepted: 16 November 2012
  •  Published: 27 February 2013

Abstract

 

From oil-contaminated soil, three lipase-producing microorganisms were selected as good lipase producers using rhodamine B-olive oil plate agar and they were identified as from Pseudomonas, Burkholderia and Klebsiella genera by morphology, biochemical characterization and 16S rRNA gene sequencing. Among the three strains, Pseudomonas sp. ZBC1 was found to produce thermophilic neutral lipase which exhibited maximum activity at 80ºC and pH 7.0. Plackett-Burman design (PB design) and response surface methodology (RSM) analysis showed that the optimum values of the most significant factors are MgSO4 (0.85 g/L), inoculum size (65.9 ml/L) and volume of medium (51.57 ml). Under the optimal conditions, lipase production byPseudomonas sp. ZBC1 was enhanced by approximately 2.30 fold (from 8.50 to 19.50 U/ml).

 

Key words: Pseudomonas, lipase, thermophilic, response surface methodology.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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