African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Assessment of genetic diversity in Sudanese maize (Zea mays L.) genotypes using random amplified polymorphic DNA (RAPD) markers

Atif I. Abuali1, Awadalla A. Abdelmula2 and Mutasim M. Khalafalla3*
1Desertification Research Institute, National Centre for Research, P.O. Box 2404, Khartoum, Sudan. 2Department of Agronomy, Faculty of Agriculture, University of Khartoum, 13314 Shambat, Sudan. 3Commission for Biotechnology and Genetic Engineering, National Centre for Research, P.O. Box 2404, Khartoum, Sudan.
Email: [email protected]

  •  Accepted: 17 June 2011
  •  Published: 08 August 2011

Abstract

The randomly amplified polymorphic DNA (RAPD) molecular markers were used to assess genetic diversity in 27 Sudanese maize genotypes. Ten primers were used, resulting in the amplification of 59 fragments, of which 53 (89.33) were polymorphic. The maximum number of fragment bands (10) were produced by the primer A-1 with 100% polymorphism, while the minimum numbers of fragments (3) were produced by the primer OPA-20. Using the unweighted pair group method with arithmetic averages (UPGMA) method, the genetic associations obtained showed three distinct heterotic groups. The high rate of polymorphism between genotypes revealed by RAPD markers indicated that the method is efficient to analyze genetic divergence and can be used to establish consistent heterotic groups between maize genotypes.

 

Key words: Randomly amplified polymorphic DNA (RAPD) markers, DNA polymorphism, maize genetic diversity.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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