African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Effect on osmotic fragility of red blood cells of whole blood submitted to vibrations in an oscillating platform

Milena O. B. Monteiro1,2, Nelson de Souza Pinto1,3, Pedro J. Marin4,5, Sebastião David Santos-Filho1,6* and Mario Bernardo-Filho1,7
1Departamento de Biofísica e Biometria, Instituto Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. 2Programa de Pós-Graduação em Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. 3Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil. 4Laboratory of Physiology, European University Miguel de Cervantes, Valladolid, Spain. 5Research Center on Physical Disability, ASPAYM, Castilla y León, Spain. 6Centro de Ciências da Saúde, Universidade Severino Sombra, Vassouras, RJ, Brasil. 7Coordenadoria de pesquisa, Instituto Nacional do Câncer, INCa, Rio de Janeiro, Brasil.  
Email: [email protected]

  •  Accepted: 07 September 2011
  •  Published: 26 October 2011

Abstract

Whole body vibration (WBV) exercises in oscillating platforms (OP) have emerged in sports and in the rehabilitation procedures of clinical disorders. The aim of this work was to verify the effects of vibrations on the osmotic fragility (OF) of red blood cells (RBC) isolated from whole blood submitted to OP. Heparinized blood samples were withdrawn from German dog, and distributed in tubes that were divided in three sets. A set of tubes was submitted to 0 Hz (control), the second set of tubes was submitted to 10 Hz and the third set to 20 Hz for 1 min in an OP. Then the OF of the RBC was determined, and the results indicate that the vibration promotes an increase on the hemolysis from 9 ± 1 to 20 ± 2% with 10 and 20 Hz, respectively. The shape of the OF curves for 10 and 20 Hz were similar. A significant difference (P<0.05) was found when a comparison was done with the control curve (0 Hz) and the experimental sets (10 and 20 Hz). Our findings therefore indicate that the increase of the frequency from 10 to 20 Hz seems to induce damage on the RBC membrane and this effect was dependent on the frequency. In addition, we suggest precaution with the use of the vibration generated in OP.  

 

Key words: Vibratory platform, hemolysis, frequency, erythrocyte membrane, blood.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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