African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Synthesis and characterization of 2-mercapto-N-methyl imidazole substituted benzimidazole derivatives and investigation of their effect on production of plantlets in Oncidium Gower Ramsey

Mahesh R
  • Mahesh R
  • Department of Chemistry, R.V. College of Engineering, Bangalore, India.
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Ramya K
  • Ramya K
  • Visvesvaraya Technological University, Belgaum 590018, India.
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Ashok Kumar HG
  • Ashok Kumar HG
  • Visvesvaraya Technological University, Belgaum 590018, India.
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Satyanarayana S*
  • Satyanarayana S*
  • Department of Biotechnology, R.V. College of Engineering, Bangalore, India.
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  •  Received: 23 December 2014
  •  Accepted: 30 March 2015
  •  Published: 15 April 2015

Abstract

A series of benzimidazole derivatives (5a-c) were synthesized by coupling 5-substituted 2-chloromethyl benzimidazole (3a-c) with 2-mercapto-N-methyl imidazole. The synthesized compounds were characterized by infra-red (IR), nuclear magnetic resonance (NMR) and elemental analyses. Further, the synthesized compounds were tested on plantlet production from protocorm like bodies (PLBs) sections of Oncidium. PLBs sections were cultured on half strength modified Murashige and Skoogs (MS) medium alone and also modified MS medium supplemented with the synthesized chloro, methyl and nitro derivatives individually at 2 or 5 µM concentrations. Among these three compounds, PLBs sections cultured on medium supplemented with nitro compound (5c) at 5 µM concentration produced maximum number (95) of plantlets. 
 
Key words: Benzimidazole derivatives, protocorm like bodies, plant growth regulators, 2-mercapto-N-methyl imidazole.
 

Abbreviation

PLBs, Protocorm like bodies; CMNP, 5-chloro-3-methyl-4-nitro-1H-pyrazole; ZnAAC, zinc-amino acid complexes; TIBA, 2,3,5-triiodobenzoic acid; PCIB, 2-(p-chlorophenoxy)-2-methylpropionic acid; IR, infra-red; NMR, nuclear magnetic resonance.

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