African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12083

Full Length Research Paper

Protective effect of Allium sativa extract against carbon tetrachloride- induced hepatic oxidative stress and hyperlipidemia in rats

Chikezie Paul Chidoka*
  • Chikezie Paul Chidoka*
  • Department of Biochemistry, Imo State University, Owerri, Nigeria.
  • Google Scholar
Uwakwe Augustine Amadikwa2
  • Uwakwe Augustine Amadikwa2
  • Department of Biochemistry, University of Port-Harcourt, Port-Harcourt, Nigeria.
  • Google Scholar


  •  Received: 01 October 2013
  •  Accepted: 03 April 2014
  •  Published: 09 April 2014

Abstract

Pathogenesis of several chronic liver diseases has been attributed to overwhelmed antioxidant protective system against reactive oxygen species (ROS). The present study ascertained the capacity of short-term administration of ethanolic extract of Allium sativa to neutralize ROS and ameliorate hyperlipidemia. Hyperlipidemia was induced in rats by single intra-peritoneal injection of CCl4 (dosage = 2.0 mL/kg), followed by treatment with ethanolic extract of A. sativa (dosage: 200 and 400 mg/kg) at a regular interval of 16 for 64 h. Blood samples were drawn from the rats at t = 0 h and t = 76 h, that is, 12 h after the end of 64 h treatment with CCl4/A. sativa extract, to ascertain for hepatic function and serum lipid profile (SLP). In addition, liver post mitochondrial supernatant (PMS) fraction was measured for oxidative stress indicators: lipid peroxidation (LPOx), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and reduced glutathione (GSH). On the average, short-term administration of ethanolic extract of A. sativa caused reduction of SLP in the following magnitude: total cholesterol (TC) = 19.48%, triacylglycerol (TAG) = 48.59%, VLDL-C = 48.57%, LDL-C = 19.49% and increase in HDL-C = 32.43%. Also, improvement in oxidative stress indicators gave SOD = 10.20%, GPx = 30.92%, CAT = 18.18%, LPOx = 35.92% and GSH = 51.09%. Although the administration of A. sativa extract to the rats did not restore full therapeutic benefits within the experimental time (t = 76 h), the capacity of the plant extract to ameliorate oxidative stress and hyperlipidemia in the animals was fairly at par with the standard hepatic drug-hepaticum.

 

Key words: Allium sativa, hepatocyte, hyperlipidemia, lipid profile, oxidative stress.