Abstract

Efficient plant regeneration through organogenesis was achieved from callus cultures derived from leaf explants of Elaeagnus angustifolia. Calli were obtained on MS medai containing 3% sucrose and different concentrations of TDZ (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum percentage response for callus formation was 85% on MS medium supplemented with 2 mg/l of TDZ. Shoot organogenesis was achieved after transfer of calli to MS media with 3% sucrose and different concentrations of  BA (0.5, 1, 2, 3, 4 and 6 mg/l) and kinetin (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum organogenesis was obtained with 2 mg/l of BA. Rooting of the shoots was achieved on MS supplemented with 0.2 mg/l of  IBA.  Regenerated plantlets were acclimatized and successfully transplanted to soil.

Key words:  shoot organogenesis, callus, oleaster.

Abbreviation

Abbreviations: BA, 6-Benzyladenine; 2,4-D, 2,4-Dichlorophenoxyacetic acid;  MS, Murashige and Skoog (1962) basal medium; NAA, α-naphthalene acetic accid; IBA, indole-3-butyric acid; TDZ,  N-phenyl-N0 (1,2,3-thidiazol-5-yl) urea.