African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

L-Arabinose and oligosaccharides production from sugar beet pulp by xylanase and acid hydrolysis

Rina Wu1, Huashan Zhang2, Xiaoying Zeng1, Jitai Zhang1 and Hairong Xiong1*
  1Engineering research centre for the protection and utilization of bioresource in southern China, College of life science, South-central University for Nationalities, Wuhan, 430074, China. 2Key laboratory of fermentation engineering, Ministry of education (Hubei University of Technology), Wuhan, 430068, China.
Email: [email protected]

  •  Accepted: 07 January 2011
  •  Published: 07 March 2011

Abstract

 

Xylanase and sulfuric acid were used to hydrolyze sugar beet pulp for the production of L-arabinose and oligosaccharides. Xylanase was obtained by the solid state fermentation of Thermomyces lanuginosus DSM10635. Xylanase or dilute sulfuric acid hydrolysis was adopted to hydrolyze sugar beet pulp. The hydrolysates were quantitatively identified by high-performance liquid chromatography (HPLC). The content of arabinose was 48.4% in the total monosaccharide of sulfuric acid hydrolysate, whereas, more oligosaccharides were obtained from sugar beet pulp hydrolysate of xylanase. This study has established a new way for arabinose production, as well a potential method for oligosaccharides production.

 

Key words: Xylanase, hydrolysis, sugar beet pulp, L-arabinose, HPLC.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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