African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Introduction

Genetic diversity and relationship assessment among mulberry (Morus spp) genotypes by simple sequence repeat (SSR) marker profile

Shabir A. Wani1, M. Ashraf Bhat2, G. N. Malik1, Farooq A. Zaki3, M. R. Mir1,  Nawsheeba Wani4 and Khalid Mushtaq Bhat4
1Temperate Sericulture Research Institute (TSRI), SKUAST- Kashmir, Mirgund, Jammu and Kashmir, India. 2Molecular Biology Laboratory Laborotory, Centre for Plant Biotechnology, SKUAST-Kashmir, Shalimar-191121 Jammu and Kashmir, India. 3Registrar Office, SKUAST-Kashmir, Shalimar-191121 Jammu and Kashmir, India.         4Division of Fruit science, SKUAST-Kashmir, Shalimar-191121 Jammu and Kashmir, India.  
Email: [email protected]

  •  Accepted: 08 April 2013
  •  Published: 22 May 2013

Abstract

Mulberry (Morus L.) is essential for sericulture industry as the primary source of food for silkworm Bombyx mori L. In India, long tradition of practising sericulture includes the use of a large number of indigenous cultivars. Since knowledge on genetic divergence of these cultivars/varieties is imperative for conservation and gainful utilization, simple sequence repeat (SSR) profiling was employed to assess genetic relatedness among 17 mulberry genotypes maintained in the Germplasm Bank of Temperate Sericulture Institute, SKUAST Kashmir, Mirgund. Six SSR primers were utilised which generates 17 alleles among the genotypes. The polymorphism information content (PIC) value varied from 0.260 (MulSTR3) to 0.623 (MulSTR4), with an average of 0.438 per locus. The highest similarity value of 0.92 was observed between Lemoncina and Kanva-2, as compared to the lowest similarity coefficient of 0.15 was between SKM-48 and Chinese white. Clustering of the genotypes was done with unweight pair group method using arithmetic average (UPGMA) which generates five clusters. Cluster-2 contained maximum (six) genotypes.

 

Key words: Clustering, genetic relatedness, mulberry, SSR.