African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Isolation and characterization of efficient cellulolytic fungi from degraded wood and industrial samples

Abreham Bekele*
  • Abreham Bekele*
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia; Department of Biotechnology, College of Natural and Computational Sciences, Wolkite University, Ethiopia.
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Tariku Abena
  • Tariku Abena
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia.
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Adane Habteyohannes
  • Adane Habteyohannes
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia.
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Addisalem Nugissie
  • Addisalem Nugissie
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia.
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Fitala Gudeta
  • Fitala Gudeta
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia.
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Tigist Getie
  • Tigist Getie
  • Department of Biotechnology, College of Natural and Computational Sciences, University of Gondar, Gondar, Ethiopia.
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Musin Kelel
  • Musin Kelel
  • Department of Biotechnology, School of Science and Technology, Addis Ababa Science and Technology University, Ethiopia.
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Admas Berhanu
  • Admas Berhanu
  • Department of Biotechnology, College of Natural and Computational Sciences, Wolkite University, Ethiopia.
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  •  Received: 27 April 2015
  •  Accepted: 02 June 2015
  •  Published: 02 December 2015

Abstract

Cellulose is the most abundant biopolymer and renewable natural product in the biosphere. Cellulose degrading fungal strains play an important role in recycling of cellulosic materials. They have immense advantage in various industries to hydrolyze cellulosic substrates for production of various products. This study was thus aimed to isolate and characterize efficient cellulose degrading fungi from their common natural habitats. Decaying Acacia wood and industrial water effluent samples were used for isolation and screening of cellulolytic fungi. Both samples were serially diluted and cultured on cellulose basal medium (CBM) supplemented with 30 mg/L chloramphenicol as bactericidal agent. Cellulose degrading fungal isolates were selected based on their hydrolyzed zone after congo red dye stain. Among 13 initial isolates, four isolates (C, E, G, and H) were finally screened as the most efficient fungal isolates representing only degraded Acacia tree. These isolates were confirmed as Penicillium species (C), Apergillus terrus (G), Alternaria species (H) and Apergillus species (E). From this study, the decaying Acacia sample was found to be the best source for cellulolytic fungi than that of wastewater sample. Out of these isolates, the maximum zone of hydrolysis (51.33±1.53 mm) was obtained for ‘isolate E’, whereas the minimum zone of clearance (26.67±1.53 mm) was recorded for penicillium species. This study indicates the existence of potential cellulolytic fungal on decayed wood of Acacia. Hence, further molecular aided characterizations of the isolates and their enzymes are of paramount importance for their use for industrial purposes.

Key words: Cellulase, cellulosic basal medium, congored, fungi, zone of hydrolysis.