African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

In vitro regeneration of Acacia nilotica from nodes on MS medium

Samake Gabriel1, Traore Bakary M.2, Senou Hamidou3, Wang Hua-Fang1*
  1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People’s Republic of China. 2The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing100083, People’s Republic of China. 3Plant Biotechnology Laboratory of the Rural Polytechnic Institute of Training and Applied Research of Katibougou, Rep. Mali.
Email: [email protected]

  •  Accepted: 17 June 2011
  •  Published: 21 September 2011

Abstract

 

In the regeneration of Acacia nilotica, the effect of cytokinin (BA) and auxin (IBA) in factorial experimentation on the MS medium was determined with different dosages each. Starting from the epicotyls of germinated seeds, a combination of benzylaminopurin, naphtaleneacetic acid and gibberelic acid (BA*NAA*GA3) served as a good initiator of adventitious buds. However, the best production of nodes was obtained with BA for 0.1 mgl-1 dosage. The rate of node production was equal to 6.767%, while the longest stem was 53.33 mm. The best and high rate of rooting was 93.33 and 83.33%, respectively with 0.1 and 1.0 mg/l of IBA. The interaction between the two hormones had a negative effect on the growth and rooting of the shoots, whilst high dosage had a shortening effect on the nodes.

 

Key words: Acacia nilotica, regeneration, microcutting, rooting, growth, cotyledonary node.

Abbreviation

BA, Benzylaminopurin; IBA, indol-3-butyric acid; NAA, naphtalenacetic acid; GA, gibberelic acid.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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