African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Full Length Research Paper

Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid

  Hooi Wei Yeng1, Mariana Nor Shamsudin2,3 and Raha Abdul Rahim1,2*  
  1Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia 43400 UPM Serdang Selangor, Malaysia. 2Institute of Biosciences, Universiti Putra Malaysia 43400 UPM Serdang Selangor, Malaysia. 3Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 11 June 2009
  •  Published: 02 November 2009

Abstract

 

To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis.

 

Key words: Plasmid vector, transformation, expression.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 21
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES