African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Full Length Research Paper

Morphology and histochemistry of primary flight muscles in Rhinolophus mehelyii

Suna Cebesoy
Ankara University, Faculty of Science, Deparment of Biology, 06100 Tandogan, Ankara, Turkey.
Email: [email protected]

  •  Accepted: 13 January 2009
  •  Published: 20 March 2009

Abstract

 

Two primary flight muscles of Rhinolophus mehelyii were studied using morphological and histochemical analysis. Two fast-twitch fiber types are histochemically identified in pectoralis muscles of R. mehelyii. These were classified as type IIa and type IIb according to glycine-calcium-formalin preincubation staining protocol for myosin ATPase. The primary flight muscles, serratus ventralis included type I, type IIa and type IIb fibers. Type I fibers were highly oxidative, as stained dark for NADH-TR. Type IIa fibers exhibited relatively weak staining properties for NADH-TR and SDH, indicating an intermediate oxidative capacity. Type IIb fibers showed low oxidative capacity, as indicated by weak reaction for NADH-TR.

 

Key words: Rhinolophus mehelyii, skeletal muscles, ATPase, NADH-TR, SDH, histochemical tests.