Comparison of ompP5 sequence-based typing and pulsed-filed gel electrophoresis for genotyping of Haemophilus parasuis

Bin Zhang; Chenggang Xu; Jianmin Zhang; Lingyun Zhang; Lili Guo; Saixiang Feng; Ming Liao

doi:10.5897/AJB11.2937

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12487

Full Length Research Paper

Comparison of ompP5 sequence-based typing and pulsed-filed gel electrophoresis for genotyping of Haemophilus parasuis

Bin Zhang

Bin Zhang
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Chenggang Xu

Chenggang Xu
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Jianmin Zhang

Jianmin Zhang
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Lingyun Zhang

Lingyun Zhang
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Lili Guo

Lili Guo
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Saixiang Feng

Saixiang Feng
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Ming Liao

Ming Liao
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou510642, People's Republic of China.
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Article Number - 8834C0D39072
Vol.11(22), pp. 6004-6010 , March 2012
https://doi.org/10.5897/AJB11.2937

Accepted: 13 December 2011
Published: 15 March 2012

Abstract

In this study, comparison of the outer membrane protein P5 gene (ompP5) sequence-based typing with pulsed-field gel electrophoresis (PFGE) for the genotyping of Haemophilus parasuis, the 15 serovar reference strains and 43 isolates were investigated. When comparing the two methods, 31 ompP5 sequence types (STs) and 43 PFGE types were identified withompP5 STs A16 and A28 being the dominant ompP5 ST types, while no predominant PFGE type was found. The discriminatory indices were 0.95 for ompP5 sequence-based method and 0.98 for PFGE, and the two techniques were proved to be 70.7% congruent. Therefore,ompP5 sequence-based typing was a simple and inexpensive method, enabling it as a preliminary technique to research the molecular differentiation of H. parasuis.