African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

A new method for promoting lily flowering

Shuang Li1,2, Xia Ren1, Conglin Huang1, Zhongyi Wu1, Guixia Jia2* and Xiuhai Zhang1*
1Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China. 2College of Landscape Architecture, National Engineering Research Center for Floriculture, Beijing Forestry University, China.
Email: [email protected], [email protected]

  •  Accepted: 06 April 2011
  •  Published: 08 August 2011

Abstract

FT is thought to be the florigen in plants. In this research, a new method for promoting lily flowering was introduced. The function of FT gene cloned fromArabidopsis on promoting lily flowering was analyzed. pET-30a-FT vector was constructed to indicate the expression of FT:eGFP fuse protein in prokaryotic cells. FT:eGFP was also constructed into plant virus vector-pGR106. AgrobacteriumGV3101 harboring pGR106-FT was injected into lily. The injected lily showed early flowering when compared with the control plants. The detection of eGFP and PCR analysis indicated that the virus harboring FT:eGFP was replicated and expressed in the host plants. The results showed that FT:eGFP fuse protein functioned in promoting lily flowering.

 

Key words: FT, viral vector, lily.