African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Optimization of the technology of extracting water-soluble polysaccharides from Morus alba L. leaves

Zhonghai Tang1, Shiyin Guo1, Liqun Rao1, Jingping Qin1, Xiaona Xu3 and Yizeng Liang2*
1College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128 ,China. 2Research Center of Modernization of Chinese Traditional and Herbal Drug Modernization, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan,P. R. China. 3College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, China.
Email: [email protected]

  •  Accepted: 19 May 2011
  •  Published: 03 October 2011

Abstract

To optimize the parameters for extracting water-soluble polysaccharides from mulberry leaves using hot water, the extraction process was optimized by the orthogonal test through the single-factor experiment. Experiments were carried out using an L9 (34) orthogonal design to examine the effects of extraction temperature, extraction duration, concentration of the material and concentration of ethanol on the polysaccharide yield. The optimum extraction conditions determined were as follows: concentration of material was equal to 1:24, extraction temperature was 70°C, extraction duration was 90 min and concentration of ethanol was equal to 80%. Under these conditions, the yield of polysaccharides was 2.64%.

 

Key words: Polysaccharides, Morus alba L., extraction technology, single-factor experiment, orthogonal test.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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